LOCUS       40924_3886        6398 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Drosophila transfection vector pact-MCS DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Ito H, Sato K, Koganezawa M, Ote M, Matsumoto K, Hama C, Yamamoto D.
  TITLE     Fruitless recruits two antagonistic chromatin factors to establish 
            single-neuron sexual dimorphism
  JOURNAL   Cell 149 (6), 1327-1338 (2012)
  PUBMED    22682252
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Ito H, Yamamoto D.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2012) Contact:Hiroki Ito Tohoku University, 
            Graduate School of Life Sciences; 2-1-1 Katahira, Aoba-ku, Sendai, 
            Miyagi 9808577, Japan
REFERENCE   3  (bases 1 to 6398)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6398)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "2012"; volume: "149"; issue: "6"; pages: "1327-1338"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-SEP-2012) Contact:Hiroki Ito Tohoku University, Graduate School 
            of Life Sciences; 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi 9808577, 
            Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        687..791
                     /label=AmpR promoter
     CDS             792..1649
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1822..2410
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2698..2719
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2734..2764
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2772..2788
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2796..2812
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2871..5405
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     misc_feature    5417..5460
                     /note="multiple cloning site; 
                     EcoRI-BamHI-XbaI-NotI-SacI-KpnI-SalI"
     misc_feature    5461..6398
                     /note="3'region containing polyA signal of Drosophila
                     Act5C"
ORIGIN
        1 ctgcagattg tttagcttgt tcagctgcgc ttgtttattt gcttagcttt cgcttagcga
       61 cgtgttcact ttgcttgttt gaattgaatt gtcgctccgt agacgaagcg cctctattta
      121 tactccggcg ctcttttcgc gaacattcga ggcgcgctct ctcgaaccaa cgagagcagt
      181 atgccgttta ctgtgtgaca gagtgagaga gcattagtgc agagagggag agacccaaaa
      241 agaaaagaga gaataacgaa taacggccag agaaatttct cgagttttct ttctgccaaa
      301 caaatgacct accacaataa ccagtttgtt ttgggattct aggggaatta attcgcctga
      361 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
      421 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
      481 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
      541 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
      601 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      661 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      721 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      781 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      841 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      901 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      961 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1021 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1081 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1141 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1201 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1261 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1321 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1381 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1441 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1501 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1561 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1621 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1681 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1741 ataatctcat gaccaaaatc cctaacgtga gttttcgttc cactgagcgt cagaccccgt
     1801 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1861 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1921 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     1981 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2041 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2101 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2161 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2221 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2281 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2341 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2401 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2461 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     2521 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     2581 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     2641 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2701 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2761 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2821 cgaattaatt ctatattcta aaaacacaaa tgatacttct aaaaaaaaat catgaatggc
     2881 atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact gtcacatcat
     2941 ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt tatggccatt
     3001 aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac attcgagaaa
     3061 gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga aggagaacga
     3121 gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat attcggagca
     3181 atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat gtttttcacc
     3241 gttcccgggg ccattttcaa agactcgtcg gtaagataag attgtgtcac tcgctgtctc
     3301 tcttcatttg tcgaagaatg ctgaggaatt tcgcgatgac gtcggcgagt attttgaaga
     3361 atgagaataa tttgtattta tacgaaaatc agttagtgga attttctaca aaaacatgtt
     3421 atctatagat aattttgttg caaaatatgt tgactatgac aaagattgta tgtatatacc
     3481 tttaatgtat tctcattttc ttatgtattt ataatggcaa tgatgatact gatgatattt
     3541 taagatgatg ccagaccaca ggctgatttc tgcgtctttt gccgaacgca gtgcatgtgc
     3601 ggttgttgtt ttttggaata gtttcaattt tcggactgtc cgctttgatt tcagtttctt
     3661 ggcttattca aaaagcaaag taaagccaaa aaagcgagat ggcaatacca aatgcggcaa
     3721 aacggtagtg gaaggaaagg ggtgcggggc agcggaagga agggtggggc ggggcgtggc
     3781 ggggtctgtg gctgggcgcg acgtcaccga cgttggagcc actcctttga ccatgtgtgc
     3841 gtgtgtgtat tattcgtgtc tcgccactcg ccggttgttt ttttcttttt atctcgctct
     3901 ctctagcgcc atctcgtacg catgctcaac gcaccgcatg ttgccgtgtc ctttatgcgt
     3961 cattttggct cgaaataggc aattatttaa acaaagatta gtcaacgaaa acgctaaaat
     4021 aaataagtct acaatatggt tacttattgc catgtgtgtg cagccaacga tagcaacaaa
     4081 agcaacaaca cagtggcttt ccctctttca ctttttgttt gcaagcgcgt gcgagcaaga
     4141 cggcacgacc ggcaaacgca attacgctga caaagagcag acgaagtttt ggccgaaaaa
     4201 catcaaggcg cctgatacga atgcatttgc aataacaatt gcgatattta atattgttta
     4261 tgaagctgtt tgacttcaaa acacacaaaa aaaaaaataa aacaaattat ttgaaagaga
     4321 attaggaatc ggacagctta tcgttacggg ctaacagcac accgagacga aatagcttac
     4381 ctgacgtcac agcctctgga agaactgccg ccaagcagac gatgcagagg acgacacata
     4441 gagtagcgga gtaggccagc gtagtacgca tgtgcttgtg tgtgaggcgt ctctctcttc
     4501 gtctcctgtt tgcgcaaacg catagactgc actgagaaaa tcgattacct attttttatg
     4561 aatgaatatt tgcactatta ctattcaaaa ctattaagat agcaatcaca ttcaatagcc
     4621 aaatactata ccacctgagc gatgcaacga aatgatcaat ttgagcaaaa atgctgcata
     4681 tttaggacgg catcattata gaaatgcttc ttgctgtgta cttttctctc gtctggcagc
     4741 tgtttcgccg ttattgttaa aaccggctta agttaggtgt gttttctacg actagctagt
     4801 gatgccccta ctagaagatg tgtgttgcac aaatgtccct gaataaccaa tttgaagtgc
     4861 agatagcagt aaacgtaagc taatatgaat attatttaac tgtaatgttt taatatcgct
     4921 ggacattact aataaaccca ctataaacac atgtacatat gtatgttttg gcatacaatg
     4981 agtagttggg gaaaaaatgt gtaaaagcac cgtgaccatc acagcataaa gataaccagc
     5041 tgaagtatcg aatatgagta acccccaaat tgaatcacat gccgcaactg ataggaccca
     5101 tggaagtaca ctcttcatgg cgatatacaa gacacacaca agcacgaaca cccagttgcg
     5161 gaggaaattc tccgtaaatg aaaacccaat cggcgaacaa ttcataccca tatatggtaa
     5221 aagttttgaa cgcgacttga gagcggagag cattgcggct gataaggttt tagcgctaag
     5281 cgggctttat aaaacgggct gcgggaccag ttttcatatc actaccgttt gagttcttgt
     5341 gctgtgtgga tactcctccc gacacaaagc cgctccatca gccagcagtc gtctaatcca
     5401 gagaccccgg atctgcgaat tcggatcctc tagagcggcc gcgagctcgg taccgtcgac
     5461 catgaagatc aagatcattg ccccgccaga gcgcaagtac tctgtctgga tcggtggctc
     5521 catcctggct tcgctgtcca ccttccagca gatgtggatc tccaagcagg agtacgacga
     5581 gtccggcccc tccattgtgc accgcaagtg cttctaagaa ggatcgcttg tctgggcaag
     5641 aggatcagga tcgggatggt cttgattctg ctggaggagg aggaggagaa gtcgaggaag
     5701 cagcagcgaa agtgcaagtg cgagtggtgg aagtttggag tgcagcacaa caaaatcaac
     5761 aacaacacca actacaagat gaaaagagcg gaaccacctg cacaccatca tcactatcat
     5821 catcgttttg ggcgcatgtt gtgtggttcc agcgtattaa tataattaat ttattccaca
     5881 tgagatatga tatgatatac tatgtatttt ttgttttttt tttatttgta aacctttaat
     5941 ataacaagaa ctacaaaaaa gtgaaaatga gcgaaaatgc atattctgcc attccacaca
     6001 cacaccaaca acacccaaca cacgcacacc cacaagctta cacacacaca cattcgcggc
     6061 atgacaagga catcaagata aagaagaact taaagaagat atttcccaaa gcgcaaaaag
     6121 aacacacaca cattgcaaaa cacaaacaac acactagcgt tttgtacaat tcgtcagcaa
     6181 ccttatgtat tattttttaa ttatgatgta attataaaca aagtgaaaca aaaatatgaa
     6241 aacaaaaagg aaaatcaaat ctgtcttctc tttctcccgc tctcctcgct ctctgctgct
     6301 aacctcgccc tctcctctct catctttttg tctgtctctc ttcacatttt tgccggccgg
     6361 caaaataata acccacacac actcacactt ggctgcag
//
